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turkey b cell line (ATCC)

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ATCC turkey b cell line
Turkey B Cell Line, supplied by ATCC, used in various techniques. Bioz Stars score: 92/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mdtc+rp19/MDTC-RP19/pm40143230-70-1-5
Average 92 stars, based on 9 article reviews

turkey b cell line - by Bioz Stars, 2026-09

92/100 stars

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Isolation:

Article Title: A Novel Adenovirus in Chinstrap Penguins ( Pygoscelis antarctica ) in Antarctica
Article Snippet: .. For isolation attempts of CSPAdV-1, 5% (w/v) kidney homogenates of virus-infected penguins (CSP10-2 and CSP10-3) were inoculated onto MDTC-RP19 (ATCC® CRL-8135TM), a lymphoblastoid cell line of turkey origin ( Meleagris gallopavo ) with high susceptibility to TAdV-3, a member of the genus, Siadenovirus [ ]. ..

Article Title: A Novel Adenovirus in Chinstrap Penguins ( Pygoscelis antarctica ) in Antarctica
Article Snippet: .. Isolation of CSPAdV-1 was attempted by inoculating the MDTC-RP19 (ATCC® CRL-8135TM) cell line [ ] with kidney homogenates, but all such attempts were unsuccessful. ..

Article Title: A Novel Adenovirus in Chinstrap Penguins ( Pygoscelis antarctica ) in Antarctica
Article Snippet: .. Virus isolation attempts from kidney homogenates in the MDTC-RP19 (ATCC® CRL-8135TM) cell line were unsuccessful. ..

Virus:

Article Title: Real-time PCR-based infectivity assay for the titration of turkey hemorrhagic enteritis virus, an adenovirus, in live vaccines.
Article Snippet: The current in vitro titration method for turkey hemorrhagic enteritis virus (THEV) is the end-point dilution assay (EPD) in suspension cell culture (CC).. This assay is subjective and results in high variability among vaccine lots.. In this study, a new in vitro infectivity method combining a SYBR Green I-based qPCR assay and CC was developed for titration of live hemorrhagic enteritis (HE) CC vaccines.

Infection:

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Fig. 1. Sialic acid is abundantly expressed on RP19 cells and is sensitive to neuraminidase treatment. (a) The expression of sialic acids on RP19 cells was verified by the staining with FITC-conjugated wheat germ agglutinin (WGA) and flow-cytometric analysis. Stained cells were incubated with 1 µg FITC-WGA, while unstained cells were incubated with PBS only. (b) RP19 cells are sensitive to neuraminidase treatment as shown by sialic acid release post-treatment. Cells were incubated with 10 mU neuraminidase from either V. cholerae (VCN) or C. perfringens (CPN) under either acidic (pH 5.25) or neutral (pH 7.4) conditions and the released sialic acid was measured at the indicated time points post-treatment. Untreated cells were used for background subtraction at each time point.

Journal: The Journal of general virology

Article Title: Turkey adenovirus 3, a siadenovirus, uses sialic acid on N -linked glycoproteins as a cellular receptor.

doi: 10.1099/jgv.0.001429

Figure Lengend Snippet: Fig. 1. Sialic acid is abundantly expressed on RP19 cells and is sensitive to neuraminidase treatment. (a) The expression of sialic acids on RP19 cells was verified by the staining with FITC-conjugated wheat germ agglutinin (WGA) and flow-cytometric analysis. Stained cells were incubated with 1 µg FITC-WGA, while unstained cells were incubated with PBS only. (b) RP19 cells are sensitive to neuraminidase treatment as shown by sialic acid release post-treatment. Cells were incubated with 10 mU neuraminidase from either V. cholerae (VCN) or C. perfringens (CPN) under either acidic (pH 5.25) or neutral (pH 7.4) conditions and the released sialic acid was measured at the indicated time points post-treatment. Untreated cells were used for background subtraction at each time point.

Article Snippet: MDTC- RP19, B- lymphoblastoid cell line (referred to as RP19 cells) was obtained from ATCC.

Techniques: Expressing, Staining, Incubation

Fig. 2. Removing or blocking of sialic acids on RP19 cells by treatment with neuraminidase or wheat germ agglutinin (WGA), respectively, reduces TAdV-3 infection. RP19 cells were incubated with neuraminidases from V. cholerae (VCN) (a), A. ureafaciens (AUN) (b) or C. perfringens (CPN) (c); or with WGA lectin (d) at the concentrations shown, followed by virus inoculation at an m.o.i. of 50 (a, c) or 10 (b, d). Virus titre was measured by qPCR-based infectivity assay. Final results are expressed as percent relative infectivity in relation to virus infectivity of untreated cells, which was normalized to 100 %. Virus titers in untreated controls are 3.88×107±8.24×106 infectious virus particles (IVP) (a), 4.16×106±3.38×106 IVP (b), 3.03×107±2.76×107 IVP (c) or 1.02×107±3.28×106 IVP (d). Columns represent means and error bars indicate standard deviations from two (a, b) or three (c, d) independent experiments each performed with duplicate (a, c) or triplicate (b, d) samples treated individually [n=4, 6, 6 and 9 for (a–d), respectively]. (e) Quantification of sialic acid residues on RP19 cells after treatment with VCN or AUN at the concentrations shown. Post-treatment, cells were stained with WGA-FITC followed by FACS analysis. NS, not significant.

Journal: The Journal of general virology

Article Title: Turkey adenovirus 3, a siadenovirus, uses sialic acid on N -linked glycoproteins as a cellular receptor.

doi: 10.1099/jgv.0.001429

Figure Lengend Snippet: Fig. 2. Removing or blocking of sialic acids on RP19 cells by treatment with neuraminidase or wheat germ agglutinin (WGA), respectively, reduces TAdV-3 infection. RP19 cells were incubated with neuraminidases from V. cholerae (VCN) (a), A. ureafaciens (AUN) (b) or C. perfringens (CPN) (c); or with WGA lectin (d) at the concentrations shown, followed by virus inoculation at an m.o.i. of 50 (a, c) or 10 (b, d). Virus titre was measured by qPCR-based infectivity assay. Final results are expressed as percent relative infectivity in relation to virus infectivity of untreated cells, which was normalized to 100 %. Virus titers in untreated controls are 3.88×107±8.24×106 infectious virus particles (IVP) (a), 4.16×106±3.38×106 IVP (b), 3.03×107±2.76×107 IVP (c) or 1.02×107±3.28×106 IVP (d). Columns represent means and error bars indicate standard deviations from two (a, b) or three (c, d) independent experiments each performed with duplicate (a, c) or triplicate (b, d) samples treated individually [n=4, 6, 6 and 9 for (a–d), respectively]. (e) Quantification of sialic acid residues on RP19 cells after treatment with VCN or AUN at the concentrations shown. Post-treatment, cells were stained with WGA-FITC followed by FACS analysis. NS, not significant.

Article Snippet: MDTC- RP19, B- lymphoblastoid cell line (referred to as RP19 cells) was obtained from ATCC.

Techniques: Blocking Assay, Infection, Incubation, Virus, Staining

Fig. 3. Selective blocking of α−2,3- and α−2,6-linked sialic acids on RP19 cells by treatment with MAL and SNA lectins, respectively, reduces TAdV-3 infection. RP19 cells were pre-incubated with the lectins MAL (a), SNA (b) or MAA+SNA (c) at the concentrations shown, followed by virus inoculation at an m.o.i. of 10. Virus titre was measured by qPCR-based infectivity assay. Final results are expressed as percent relative infectivity in relation to virus infectivity of untreated cells, which was normalized to 100 %. Virus titers in untreated controls are 1.22×107±2.05×106 IVP (a, b) or 1.02×107±3.28×106 IVP (c). Columns represent means and error bars indicate standard deviations from two (a, b) or three (c) independent experiments each performed with triplicate samples treated individually [n=6, 6 and 9 for (a–c), respectively]. NS, not significant.

Journal: The Journal of general virology

Article Title: Turkey adenovirus 3, a siadenovirus, uses sialic acid on N -linked glycoproteins as a cellular receptor.

doi: 10.1099/jgv.0.001429

Figure Lengend Snippet: Fig. 3. Selective blocking of α−2,3- and α−2,6-linked sialic acids on RP19 cells by treatment with MAL and SNA lectins, respectively, reduces TAdV-3 infection. RP19 cells were pre-incubated with the lectins MAL (a), SNA (b) or MAA+SNA (c) at the concentrations shown, followed by virus inoculation at an m.o.i. of 10. Virus titre was measured by qPCR-based infectivity assay. Final results are expressed as percent relative infectivity in relation to virus infectivity of untreated cells, which was normalized to 100 %. Virus titers in untreated controls are 1.22×107±2.05×106 IVP (a, b) or 1.02×107±3.28×106 IVP (c). Columns represent means and error bars indicate standard deviations from two (a, b) or three (c) independent experiments each performed with triplicate samples treated individually [n=6, 6 and 9 for (a–c), respectively]. NS, not significant.

Article Snippet: MDTC- RP19, B- lymphoblastoid cell line (referred to as RP19 cells) was obtained from ATCC.

Techniques: Blocking Assay, Infection, Incubation, Virus

Fig. 4. Sodium periodate (NaIO4) treatment of RP19 cells reduces TAdV-3 infection. RP19 cells were pre-treated with NaIO4 at the concentrations shown, followed by virus inoculation at an m.o.i. of 50. Virus titre was measured by qPCR-based infectivity assay. Final results are expressed as percent relative infectivity in relation to virus infectivity of untreated cells, which was normalized to 100 %. Virus titer in untreated control is 3.16×107±2.24×107 IVP. Columns represent means and error bars indicate standard deviations from three independent experiments each performed with at least triplicate samples treated individually (n=9).

Journal: The Journal of general virology

Article Title: Turkey adenovirus 3, a siadenovirus, uses sialic acid on N -linked glycoproteins as a cellular receptor.

doi: 10.1099/jgv.0.001429

Figure Lengend Snippet: Fig. 4. Sodium periodate (NaIO4) treatment of RP19 cells reduces TAdV-3 infection. RP19 cells were pre-treated with NaIO4 at the concentrations shown, followed by virus inoculation at an m.o.i. of 50. Virus titre was measured by qPCR-based infectivity assay. Final results are expressed as percent relative infectivity in relation to virus infectivity of untreated cells, which was normalized to 100 %. Virus titer in untreated control is 3.16×107±2.24×107 IVP. Columns represent means and error bars indicate standard deviations from three independent experiments each performed with at least triplicate samples treated individually (n=9).

Article Snippet: MDTC- RP19, B- lymphoblastoid cell line (referred to as RP19 cells) was obtained from ATCC.

Techniques: Infection, Virus, Control

Fig. 5. Protease treatment, but not PDMP, reduces TAdV-3 infection in RP19 cells. Cells were pre-incubated with either 300 mU bromelain (a), 5 µg trypsin (b) or 25 µm PDMP (c), followed by virus inoculation at an m.o.i. of 50 (a, b) or 20 (c). Virus titre was measured by qPCR-based infectivity assay. Final results are expressed as percent relative infectivity in relation to virus infectivity of untreated cells, which was normalized to 100 %. Virus titers in untreated controls are 2.37×108±1.53×108 IVP (a), 3.03×106±4.2×106 IVP (b), or 2.47×107±8.32×106 IVP (c). Columns represent means and error bars indicate standard deviations from two independent experiments each performed with triplicate [(a, b) n=6] or duplicate [(c) n=4] samples treated individually. NS, not significant.

Journal: The Journal of general virology

Article Title: Turkey adenovirus 3, a siadenovirus, uses sialic acid on N -linked glycoproteins as a cellular receptor.

doi: 10.1099/jgv.0.001429

Figure Lengend Snippet: Fig. 5. Protease treatment, but not PDMP, reduces TAdV-3 infection in RP19 cells. Cells were pre-incubated with either 300 mU bromelain (a), 5 µg trypsin (b) or 25 µm PDMP (c), followed by virus inoculation at an m.o.i. of 50 (a, b) or 20 (c). Virus titre was measured by qPCR-based infectivity assay. Final results are expressed as percent relative infectivity in relation to virus infectivity of untreated cells, which was normalized to 100 %. Virus titers in untreated controls are 2.37×108±1.53×108 IVP (a), 3.03×106±4.2×106 IVP (b), or 2.47×107±8.32×106 IVP (c). Columns represent means and error bars indicate standard deviations from two independent experiments each performed with triplicate [(a, b) n=6] or duplicate [(c) n=4] samples treated individually. NS, not significant.

Article Snippet: MDTC- RP19, B- lymphoblastoid cell line (referred to as RP19 cells) was obtained from ATCC.

Techniques: Infection, Incubation, Virus

Fig. 6. Tunicamycin, but not benzylGalNAc reduces TAdV-3 infection in RP19 cells. Cells were pre-incubated with tunicamycin (a) or benzylGalNAc (b) at the concentrations shown, followed by virus inoculation at an m.o.i. of 10. Virus titre was measured by qPCR-based infectivity assay. Final results are expressed as percent relative infectivity in relation to virus infectivity of untreated cells, which was normalized to 100 %. Virus titers in untreated controls are 1.9×107±1.05×107 IVP (a) or 1.3×107±6.32×106 IVP (b). Columns represent means and error bars indicate standard deviations from three [(a) n=9] or four [(b) n=12] independent experiments each performed with triplicate samples treated individually.

Journal: The Journal of general virology

Article Title: Turkey adenovirus 3, a siadenovirus, uses sialic acid on N -linked glycoproteins as a cellular receptor.

doi: 10.1099/jgv.0.001429

Figure Lengend Snippet: Fig. 6. Tunicamycin, but not benzylGalNAc reduces TAdV-3 infection in RP19 cells. Cells were pre-incubated with tunicamycin (a) or benzylGalNAc (b) at the concentrations shown, followed by virus inoculation at an m.o.i. of 10. Virus titre was measured by qPCR-based infectivity assay. Final results are expressed as percent relative infectivity in relation to virus infectivity of untreated cells, which was normalized to 100 %. Virus titers in untreated controls are 1.9×107±1.05×107 IVP (a) or 1.3×107±6.32×106 IVP (b). Columns represent means and error bars indicate standard deviations from three [(a) n=9] or four [(b) n=12] independent experiments each performed with triplicate samples treated individually.

Article Snippet: MDTC- RP19, B- lymphoblastoid cell line (referred to as RP19 cells) was obtained from ATCC.

Techniques: Infection, Incubation, Virus

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